Alexa Fluor® 488 anti-mouse/rat/human FOXP3 Antibody

Pricing & Availability
Clone
150D (See other available formats)
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
Ave. Rating
2 reviews
Product Citations
publications
150D_AF488_FOXP3_Antibody_ICFC_1_041515
C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 150D) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
  • 150D_AF488_FOXP3_Antibody_ICFC_1_041515
    C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 150D) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
  • 150D_AF488_FOXP3_Antibody_ICFC_2_041515
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
320011 25 tests $160
Check Availability


Need larger quantities of this item?
Request Bulk Quote
320012 100 tests $315
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Cross-Reactivity: Cynomolgus, Rhesus, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
  2. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol
  3. Yang ZZ, et al. 2006. Blood 107:3639.
  4. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659.
  5. Groh V, et al. 2006. Nature Immunology 7:755.
  6. Lewkowicz P, et al. 2006 J. Immunol. 177:7155.
  7. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023.
  8. Bamias G, et al. 2007. J. Immunol. 178:1809.
  9. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed
  10. Davidson TS, et al. 2007. J. Immunol. 178:4022.
  11. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249.
  12. Jaffar Z, et al. 2007. J. Immunol. 179:6193.
  13. Müller M, et al. 2007. J. Immunol. 179:2774.
  14. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed
  15. Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed
  16. Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed
  17. Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381
  18. Klunker S, et al. 2009. J. Exp Med. PubMed
  19. Haque A, et al. 2010. J. Immunol. 184:2583. PubMed
  20. Liu Y, et al. 2013. J Mol Cell Biol. PubMed
  21. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  22. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  23. Prado-Garcia H, et al. 2015. Anticancer Res.35:1529. PubMed
Product Citations
  1. Berg S 2006. J Immunol. 176:2059. PubMed
  2. Valencia X, et al. 2007. J Immunol . 178:2579. PubMed
  3. Golovina T, et al. 2008. J Immunol. 181:2855. PubMed
  4. Oida T, Weiner H 2010. PLoS One. 5:e15523. PubMed
  5. Zhu P, et al. 2012. J Cereb Blood Flow Metab. 0.482638889. PubMed
  6. Xiong Y, et al. 2012. J Biol Chem. 287:34372. PubMed
  7. Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed
  8. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  9. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  10. PRADO-GARCIA H, et al. 2015. Anticancer Res. 35:1529. PubMed
  11. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  12. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  13. Perkins T, et al. 2017. J Allergy Clin Immunol Pract. 10.1016/j.jaip.2017.01.028. PubMed
  14. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  15. Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed
  16. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_439747 (BioLegend Cat. No. 320011)
AB_439748 (BioLegend Cat. No. 320012)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.

Regulation
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID
20371 View all products for this Gene ID 317382 View all products for this Gene ID 50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Go To Top Version: 3    Revision Date: 04/15/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account