- AF6-120.1 (See other available formats)
- Other Names
- MHC class II
- Mouse (BALB/c) IgG2a, κ
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- Product Citations
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The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).Product Details
- Mouse b haplotype, cross-reacts with H-2k and H-2u haplotypes
- Antibody Type
- Host Species
- C57BL/10J splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC, IF - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).Does not react with other haplotypes (e.g., d, f, q, r, s).
(PubMed link indicates BioLegend citation)
- Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
- Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
- Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
- Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
- Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
- Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
- Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
- Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
- Product Citations
AB_493140 (BioLegend Cat. No. 116410)
- MHC class II
- B cell and activated T cells, APCs of H-2b mice
- Antigen presentation
- Ligand Receptor
- CD3/TCR, CD4
- Cell Type
- B cells, T cells, Antigen-presenting cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
- Gene ID
- 14961 View all products for this Gene ID
- View information about I-Ab on UniProt.org
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