- G8.8 (See other available formats)
- Regulatory Status
- Other Names
- CD326, EGP40, MIC18, TROP1, KSA
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- TE-71 thymic epithelial cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F, 3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.
(PubMed link indicates BioLegend citation)
- Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
- Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
- Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
- Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Ding X, et al. 2015. Cancer Res. 75:330. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_1134099 (BioLegend Cat. No. 118210)
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
- Mediates calcium-independent homophilic cell-cell adhesion.
- CD326 displays hemophilic binding.
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
- Gene ID
- 17075 View all products for this Gene ID
- View information about CD326 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD326 (Ep-CAM)
Purified anti-mouse CD326 (Ep-CAM)
Biotin anti-mouse CD326 (Ep-CAM)
PE anti-mouse CD326 (Ep-CAM)
FITC anti-mouse CD326 (Ep-CAM)
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM)
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM)
PE/Cyanine7 anti-mouse CD326 (Ep-CAM)
APC/Cyanine7 anti-mouse CD326 (Ep-CAM)
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM)
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM)
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM)
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM)
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready)
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM)
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM)
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM)
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM)
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM)
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM)
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM)
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM)
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM)
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM)
PE/Cyanine5 anti-mouse CD326 (Ep-CAM)