- 87G (See other available formats)
- Other Names
- Human Leukocyte Antigen-G
- Mouse IgG2a, κ
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- Product Citations
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HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.Product Details
- Human, African Green, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- HLA-G transfected cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.4
(PubMed link indicates BioLegend citation)
- Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
- Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
- Lila N, et al. 2002. Circulation. 105:1949.
- Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
- Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed
AB_2728295 (BioLegend Cat. No. 335917)
AB_2728296 (BioLegend Cat. No. 335918)
Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors
- Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
- ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
- Cell Type
- Dendritic cells, Epithelial cells, Macrophages, Monocytes
- Biology Area
- Molecular Family
- MHC Antigens
- Antigen References
1. Hunt JS, et al. 2005. FASEB J. 19:681.
2. Carosella ED, et al. 2008. Blood 111:4862.
- Gene ID
- 3135 View all products for this Gene ID
- View information about HLA-G on UniProt.org
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