- 5E8/CXCR2 (See other available formats)
- Other Names
- CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor
- Mouse IgG1, κ
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- Product Citations
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CXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (GPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).Product Details
- Antibody Type
- Host Species
- Human CXCR2 transfected L1.2 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.
(PubMed link indicates BioLegend citation)
- Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed.
AB_492938 (BioLegend Cat. No. 320712)
- Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1
Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
- Neutrophil activation, chemotaxis, angiogenesis
- IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-& alpha;,ß, γ (CXCL1, CXCL2, CXCL3)
- Cell Type
- Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682.
2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663.
3. Emadi S, et al. 2005. Blood 105:464.
4. Omari KM, et al. 2005. Brain 128:1003.
5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130.
6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164.
- Gene ID
- 3579 View all products for this Gene ID
- View information about CD182 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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