Alexa Fluor® 488 anti-human CD150 (SLAM) Antibody

Pricing & Availability
A12 (7D4) (See other available formats)
Regulatory Status
Other Names
SLAM, Signaling Lymphocyte Activation Molecule, IPO-3
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with A12 Alexa Fluor® 488
  • A12_Alx488_011507
    Human peripheral blood lymphocytes stained with A12 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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306312 100 tests $265
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CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Host Species
Activated human PBMC
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306317 and 306318).

Application References

(PubMed link indicates BioLegend citation)
  1. Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim)
  2. Vincent S, et al. 2002. J. Virol. 76:6121.
  3. Cocks B, et al. 1995. Nature 376:260.
  4. Sayos J, et al. 2001. Blood 97:3867. (IP)
  5. Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim)
  6. Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed
Product Citations
  1. Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
  2. Barbero AM, et al. 2021. J Leukoc Biol. 109:257. PubMed
  3. Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
AB_2187945 (BioLegend Cat. No. 306312)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, 70-95 kD

T subset (upregulated after activation), B cells, dendritic cells, endothelial cells

B cell costimulation, proliferation and Ig production
CD150 (self ligand)
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks B, et al. 1995. Nature 376:260.
2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377.
3. Polacino P, et al. 1996. J. Med. Primatol. 25:201.
4. Punnonen J, et al. 1997. J. Exp. Med. 185:993.

Gene ID
6504 View all products for this Gene ID
View information about CD150 on

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD150 (SLAM)

  • PE anti-human CD150 (SLAM)

  • Purified anti-human CD150 (SLAM)

  • Alexa Fluor® 488 anti-human CD150 (SLAM)

  • TotalSeq™-A0870 anti-human CD150 (SLAM)

  • TotalSeq™-C0870 anti-human CD150 (SLAM)

  • Ultra-LEAF™ Purified anti-human CD150 (SLAM)

  • TotalSeq™-B0870 anti-human CD150 (SLAM) Antibody

  • TotalSeq™-D0870 anti-human CD150 (SLAM)


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