Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# A488-101L)

Pricing & Availability
Clone
16B12 (See other available formats)
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# A488-101L
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16B12_AF488_HA.11_Antibody_IF_021715
HA tagged CHO cells stained with Alexa Fluor® 488 Labeled Antibody, HA.11 at 1:1000. Cells were counterstained with DAPI (blue).
  • 16B12_AF488_HA.11_Antibody_IF_021715
    HA tagged CHO cells stained with Alexa Fluor® 488 Labeled Antibody, HA.11 at 1:1000. Cells were counterstained with DAPI (blue).
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
901509 100 µl $366
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. 

Product Details
Technical data sheet

Product Details

Reactivity
YPYDVPDYA Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide, CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 0.09% Sodium Azide + 0.1% BSA.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
1 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IF, FC, ICC

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry.

The optimal working dilution should be determined for each specific assay condition.
IF: 1:1,000
FC: 1:1,000


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunofluorescence (IF) and FACS.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. HA.11 recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. This antibody was subsequently purified via a protein-G column and conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is an excellent alternative to fluorescein (FITC) dyes.

Application References

(PubMed link indicates BioLegend citation)
  1. San-Segundo PA, Roeder GS. 2000. Mol Biol Cell. 11:3601.
  2. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508.
  3. Field J, et al. 1988. Mol Cell Biol. 8:2159.
  4. Cavaretta JP, et al. 2014. PLoS One. 9:103655.
  5. Collier JH, et al. 2006. PNAS. 103:2021. (FC) PubMed
  6. Dalter C, et al. 2013. Biochim Biophys Acta. 1833:2844. (FC) PubMed
  7. Stone A, et al. 2012. J Biol CHem. 287:41139.
Product Citations
  1. Mrksich J 2006. Proc Natl Acad Sci U S A. 103:2021-2025. PubMed
  2. Grimm C 2013. Biochim Biophys Acta. 1833:2844-2855. PubMed
  3. Ketel K, et al. 2016. Nature. 529:408-412. PubMed
  4. Saloman J, et al. 2016. J Neurosci. 36: 10769 - 10781. PubMed
Publication Library
RRID
AB_2565072 (BioLegend Cat. No. 901509)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 07/26/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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