Alexa Fluor® 488 anti-GFP Antibody

Pricing & Availability
Clone
FM264G (See other available formats)
Other Names
Green Fluorescent Protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
FM264G_AF488_GFP_Antibody_FC_032514
GFP transfected CHO cells were fixed and permeabilized, and then intracellularly stained with anti-GFP (clone FM264G) Alexa Fluor® 488 (filled histogram) or isotype control rat IgG2a Alexa Fluor® 488 (open histogram).
  • FM264G_AF488_GFP_Antibody_FC_032514
    GFP transfected CHO cells were fixed and permeabilized, and then intracellularly stained with anti-GFP (clone FM264G) Alexa Fluor® 488 (filled histogram) or isotype control rat IgG2a Alexa Fluor® 488 (open histogram).
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
338007 25 tests $125.00
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338008 100 tests $265.00
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Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details
Technical data sheet

Product Details

Reactivity
GFP-tagged proteins
Host Species
Rat
Immunogen
TLR9-GFP transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Chen G, et al. J. Virol. 85:1131. PubMed
  2. Luo Y, et al. 2012. J Control Release. 162:28. PubMed
  3. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  4. Mastorakos P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8720 - 8725. PubMed
Publication Library
RRID
AB_2563287 (BioLegend Cat. No. 338007)
AB_2563288 (BioLegend Cat. No. 338008)

Antigen Details

Antigen References

1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.

Gene ID
NA
UniProt
View information about GFP on UniProt.org

Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.
Go To Top Version: 3    Revision Date: 07/07/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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