- 177.19 (See other available formats)
- Regulatory Status
- Other Names
- Autophagy protein 5
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.Product Details
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Host Species
- ATG5 peptide conjugated to KLH.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
AB_2783417 (BioLegend Cat. No. 847407)
AB_2783418 (BioLegend Cat. No. 847408)
- Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
Tissue distribution: Ubiquitous.
Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.
- ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
- ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
- Molecular Family
- Autophagosome Markers
- Antigen References
1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed
- Gene ID
- 9474 View all products for this Gene ID
- View information about ATG5 on UniProt.org
Related Pages & Pathways
Other FormatsView All ATG5 Reagents Request Custom Conjugation
|Purified anti-ATG5||177.19||WB,ICC,IHC-P,Direct ELISA|
|Alexa Fluor® 594 anti-ATG5||177.19||ICC|
|Alexa Fluor® 488 anti-ATG5||177.19||ICC|
|Alexa Fluor® 647 anti-ATG5||177.19||ICC|
|Spark YG™ 570 anti-ATG5||177.19||ICC|
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