6-His Peptide Tag (Previously Covance catalog# PEP-156P)

Pricing & Availability
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tagËäó
Previously
Covance Catalog# PEP-156P
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
931601 1 mg $181
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931602 5 mg $369
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Description

6xHis is also known as a polyhistidine-tag or His-tag.  It is commonly used as a tag to label proteins, either at the N- or C- terminus, for the purpose of purification or identification with a secondary reagent. Protein purification is typically performed using Nickel-affinity chromatography.
 

Product Details
Technical data sheet

Product Details

Purity
>90% pure by HPLC
Formulation
Lyophilized.
Storage & Handling
Store lyophilized or at -20°C in solution
Application

IP, WB

Recommended Usage

Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody

Application Notes

This synthetic peptide (H2N-HHHHHH-COOH) corresponds to the epitope detected by the 6-HIS monoclonal antibody.

This peptide can be used in competition experiments, or to elute bound 6-HIS-fusion proteins from the 6-HIS affinity matrix.

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/27/2015

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