Application: |
Flow Cytometry |
Cells used: |
Thymus, spleen, lymph node cells |
Brief Protocol: |
Extract thymus, spleen, and lymph node from euthanized mouse using tweezers and place in FACS buffer (PBS + 2% FBS + 1mM EDTA) Mash up with frosted slides and treat with Ack lysis buffer to get rid of erythrocytes. Stain cells with Fc block at 1:200 and place on ice for 5 min. Stain cells with anti-mouse CD4-647 antibody at 1:200 to 1:500 and place on ice for 20-30 min. Wash 3x with FACS buffer and complete FACS. |
Results Summary: |
Successfully detects CD4+ T cells in the thymus, lymph node and spleen. |
Additional Notes: |
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