Product: ELISA MAX™ Standard Set Mouse IL-10

Catalog No.: 431412

Trim Lajqi, PhD student FSU Jena

Measurement of IL10
Overall:	Product Quality:	Ease of Use:
Measures the production of the anti-inflammatory IL10.

Experimental Design

Application:	Measurement of IL10
Cells used:	BV2
Brief Protocol:	1. One day prior to running the ELISA, dilute Capture Antibody in 1X Coating Buffer A as described in Reagent Preparation. Add 100 μL of this Capture Antibody solution to all wells of a 96-well plate provided in this set. Seal plate and incubate overnight (16-18 hrs) between 2°C and 8°C. 2. Bring all reagents to room temperature (RT) prior to use. 3. Wash plate 4 times with at least 300 μL Wash Buffer per well and blot residual buffer by firmly tapping plate upside down on absorbent paper. All subsequent washes should be performed similarly. 4. To block non-specific binding and reduce background, add 200 μL 1X Assay Diluent A per well. 5. Seal plate and incubate at RT for 1 hour with shaking on a plate shaker. 6. While plate is being blocked, prepare the appropriate sample dilutions (if necessary) and standards. Wash plate 4 times with Wash Buffer. 8. Add 100 μL/well of standards or samples to the appropriate wells. If dilution is required, samples should be diluted in 1X Assay Diluent A before adding to the wells. 9. Seal plate and incubate at RT for 2 hours with shaking. 10. Wash plate 4 times with Wash Buffer. 11. Add 100 μL of diluted Detection Antibody solution to each well, seal plate and incubate at RT for 1 hour with shaking. 12. Wash plate 4 times with Wash Buffer. 13. Add 100 μL of diluted Avidin-HRP solution to each well, seal plate and incubate at RT for 30 minutes with shaking. 14. Wash plate 5 times with Wash Buffer. 15. Add 100 μL Substrate Solution. 16. Stop reaction by adding 100 μL of Stop Solution to each well. 17. Read absorbance at 450 nm within 15 minutes.
Results Summary:	The production of IL10 as anti-inflammatory cytokine.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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