Product: APC/Cyanine7 anti-human CD16 |
Catalog No.: 302018 |
Anne Rissiek, Dr. Universitätsklinikum Hamburg-Eppendorf (UKE) |
Anti-human CD16 APC/Cyanine7 | |||
Overall: | Product Quality: | Ease of Use: | |
Nice discrimination of neutrophils in human whole blood. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human EDTA blood |
Brief Protocol: | 1. 100 µl EDTA blood plus 50 µl antibody cocktail (in FACS Buffer), incubate for 30 min at RT. 2. Lyse red good cells by adding 1 ml Lysis Buffer, incubate for 10 min at RT, wash 2x with PBS, 400 g, 5 min, trash supernatant. 3. Resuspend cells in 200-300 µl FACS Buffer, FACS analysis The CD38 antibody was titrated and applied at a final dilution of 1:100. |
Results Summary: | Nice discrimination of neutrophils in human whole blood. |
Additional Notes: | Gating strategy: Lymphocytes --> Single cells --> CD45+ --> CD16+. |
|
|
See all Reviews |
Follow Us