Product: PE anti-mouse IL-4 |
Catalog No.: 504104 |
Minghua Zhu, Scientist Duke University |
Good Intracellular Cytokine Antibody (PE anti-mouse IL-4) | |||
Overall: | Product Quality: | Ease of Use: | |
Good antibody with strong signal, PE-IL4 is the one of the best for IL4 staining. |
Experimental Design |
Application: | Intracellular staining for cytokine production |
Cells used: | Mouse splenocytes |
Brief Protocol: | Splenocytes were stimulated for 4 h with PMA and ionomycin before intracellular staining for cytokine production. For intracellular staining, splenocytes were stained for surface markers, fixed, permeabilized, and stained for IL-17, IL-4, IL-2, IFN-γ, Good antibody combination is FITC-IL17A(#506908), PE-IL4(#504104), PECyanine5-IL2(#503824), and APC-IFNγ(#505810). |
Results Summary: | Good result every time using these antibody combination. |
Additional Notes: | We use 1:50 dilution for this antibody. |
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