Product: PE anti-mouse IL-17A

Catalog No.: 506903

Rissiek Björn, Dr. University Medical Center Hamburg-Eppendorf.

Detection of IL-17A in in vitro Generated TH17
Overall:	Product Quality:	Ease of Use:
In vitro polarized TH17 cells express IL-17A.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse CD4 T cells
Brief Protocol:	We isolated cells from spleen and peripheral lymph nodes of C57BL/6 mice and purified CD4 T cells by negative selection. Cells were polarized on CD3 precoated 24-well plates in the presence of anti-mouse CD28 (clone 37.51), anti-mouse IL-4, anti-mouse IFN-γ, mouse IL-6, human TGF-β1 and mouse IL-23 for 3 days. We restimulated cells with PMA/ionomycin and analysed IL-17 expression by intracellular cytokine staining using PE anti-mouse-IL-17A.
Results Summary:	Polarization conditions induce robust expression of IL-17A in a large fraction of CD4 T cells.
Additional Notes:

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