||9 um cryostat cut fixed frozen sections
||Tissue was rehydrated and then steamed in citrate buffer for 10 minutes to allow for mouse on mouse staining.
Blocked with donkey serum block, non-fat dry milk, and BSA.
1 hour RT incubation with primary mTuj1 at 1:400 dilution.
1 hour RT incubation with 1:150 Alexa Fluor 647 donkey anti-mouse antibody.
||Excellent staining of immature neurons, both cell body, axons in the nerve bundle, and apical dendritic processes.