Product: Purified anti-mouse CD8a |
Catalog No.: 100702 |
Robert Yuan, Graduate Student Stanford |
CD8+ T-cells are Clearly Stained with the Antibody | |||
Overall: | Product Quality: | Ease of Use: | |
The antibody stains CD8 T-cells in secondary lymphoid structures in the colon. |
Experimental Design |
Application: | Immunofluorescence |
Cells used: | Pre-fixed frozen section of a mouse colon |
Brief Protocol: | The colon was harvested from a mouse and fixed in paraformaldehyde prior to embedding in cutting medium. A section was then obtained and blocked with 5% goat serum then stained with the anti-CD8a antibody overnight. An Alexa Fluor® 488-conjugated secondary antibody was used to visualize the stain. |
Results Summary: | The antibody stains CD8 T-cells in the colon well. |
Additional Notes: |
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