Application: |
Flow Cytometry |
Cells used: |
single-cell suspension of mouse small intestinal cells |
Brief Protocol: |
Small intestine was harvested from a mouse and digested using Liberase TL to achieve a single-cell suspension. Cells were then blocked with Fc Block, stained with antibodies, and read on a flow cytometer. |
Results Summary: |
The anti-CD103 antibody stains the dendritic cells well, allowing for separation of the different subsets. |
Additional Notes: |
The shown plot has been pre-gated on CD11c-hi MHC II+ cells. |
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