Product: Purified anti-mouse CD16/32

Catalog No.: 101302

yougang zhai, research associate Loyola University Chicago

Using Fc Blocking Antibody for FACS
Overall:	Product Quality:	Ease of Use:
The blocking antibody works very well.

Experimental Design

Application:	Block cell Fc receptor before staining with cell surface marker for FACS.
Cells used:	Mouse freshly isolated peritoneal cells
Brief Protocol:	Mice were pretreated with PBS or drug (LF fraction 10). Two days later, mice were injected with LPS via i.p. and peritoneal cells were collected 4 hr later. Cells were blocked with Fc blocking antibody and then stained with FITC-anti mouse Ly-6G. cells were analyzed by FACS.
Results Summary:	Fc blocking results in highly specific staining and high fluorescence signal of neutrophil cell marker.
Additional Notes:	We used Fc blocking antibody before staining target protein in FACS and the results showed that antibody staining was very specific, suggesting that Fc blocking antibody worked very well.

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Publication Status This data has not been published.

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