Application: |
Flow Cytometry |
Cells used: |
Peripheral Blood Mononuclear Cells |
Brief Protocol: |
Isolate peripheral blood from mice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Stain with isotype control or CD19 antibody (0.4 ug/mL in FACS buffer, 1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Lyse red blood cells. Resuspend cells in 100-250 uL FACS buffer of 1.5% paraformaldehyde.
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Results Summary: |
Clearly and consistently labels CD19+ peripheral blood mononuclear cells. |
Additional Notes: |
This clone (6D5) works the best of all other BioLegend mouse CD19 clones. |
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