Application: |
Multiplex (chemokine detection from human allergic patients) |
Cells used: |
Human plasma |
Brief Protocol: |
Before loading samples on plates, plasma was centrifuged at maximum speed for 10 minutes, 4 degrees C to remove possible debris and prevent clogging. Plates were washed with vacuum manifold. Other than that, followed directions in the booklet provided with the kit exactly. Everything was done in a single day (although a long one), including running samples on flow cytometer (BD LSR II). |
Results Summary: |
Detected most chemokines in the panel at predicted concentrations, some chemokines were elevated in patients with allergies. |
Additional Notes: |
Chemokines were detected at concentrations comparable to EMD Millipore Luminex magnetic kits that ran previously, results were consistent too. |
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