Product: APC anti-human CD184 (CXCR4) |
Catalog No.: 306509 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining Transduced Human Stromal Cells With anti-CXCR4 APC. | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-CXCR4 APC works well on human stromal cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Transduced Human Mesenchymal Stromal Cells |
Brief Protocol: | Transduced human MSC were stained for 15 minutes at room temperature with the recommended dilution of anti-CXCR4 APC in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II. |
Results Summary: | CXCR4 staining was excellent. |
Additional Notes: |
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