Product: Recombinant Mouse IFN-γ (carrier-free) |
Catalog No.: 575306 |
Vidhi Chandra, Research Tech II Children's Hospital of Philadelphia |
Using CpG + IFNγ for Stimulation of Mutu Cells | |||
Overall: | Product Quality: | Ease of Use: | |
Used IFNg in conjunction with CpG to promote maturation of Mutu cells |
Experimental Design |
Application: | Bioassay |
Cells used: | Mutu (Murine DC-like cell line) |
Brief Protocol: | Treated normal Mutu cells, Mutu cells with Cofilin knockdown and Mutu cells with Fascin knockdown with CpG + IFNg to promote maturation. Knockdown of proteins was performed using lentivirus. Immature Mutu cells do not express Fascin. |
Results Summary: | Fascin staining was observed for cells treated with CpG + IFNg. |
Additional Notes: |
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