Product: APC anti-human CD45 |
Catalog No.: 304011 |
Kilian Zilkens, PhD student Yumab GmbH |
Good CD45 Antibody for Flow Cytometry | |||
Overall: | Product Quality: | Ease of Use: | |
A well distinguishable signal was received staining human PBMCs. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | human PBMCs |
Brief Protocol: | Human PBMCs were isolated from an EDTA blood sample via density gradient centrifugation. 1 x 10^6 Cells were stained on ice in the dark for 30 min. Cells were spun down, taken up in 200 µl FC-buffer (1 x PBS + 1 mM EDTA + 2 % FCS) and analysed in a Beckman Coulter CytoFlex. |
Results Summary: | A clear positive CD45 staining was seen. As expected for PBMCs, almost all cells were leucocyte marker (CD45) positive. |
Additional Notes: |
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