Application: |
Flow Cytometry |
Cells used: |
Cell suspensions from mouse lymphoid tissues such as thymus, spleen and lymph nodes |
Brief Protocol: |
We isolated cells from peripheral lymphoid tissues. Here, spleen from C57BL/6 mice, and cells were stained using APC-anti-mouse TCR Vγ1.1 antibody. We usually put up to 5 x 10^6 cells in a 50ul volume and add APC-anti-mouse TCR Vγ1.1 diluted at 1/200 final in FACS buffer for 1h at room temperature. |
Results Summary: |
APC-anti-mouse TCR Vγ1.1 provides a strong signal that allows reliable staining to study gd T cells. |
Additional Notes: |
Figure shows results from splenocytes gated in gamma delta T cells from wild type C57BL6 mice. |
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