Product: MojoSort™ Human CD4 T Cell Isolation Kit
Catalog No.: 480010
Dejan Mesner, PhD student UCL

MojoSort™ Human CD4 T Cell Isolation Kit - Excellent CD4+ T Cell Isolation (titrated)
Overall: Product Quality: Ease of Use:
Very good isolation (purity and yield) was achieved using the MojoSort kit and MACS LS columns.
Experimental Design

Application: Primary cell isolation
Cells used: PBMC from buffy coat
Brief Protocol: PBMCs were isolated from buffy coats by Ficoll density centrifugation and cultured in IL-2 media overnight.
CD4 T cells were isolated following the manufacturer's protocol: Negative Selection Protocol for the use MojoSort™ Reagents in Columns.
Two antibody dilutions were tested: 1:2 with 1:5 dilution of SA-beads, and 1:4 with 1:10 dilution of SA-beads. Columns used were MACS LS columns.
After the last wash cells were stained with CD3 and CD4 Ab (along with a pre-sort sample) and acquired on BD LSRII.
Results Summary: Very good purity achieved with both antibody dilutions, so 1:4 dilution recommended.
Additional Notes: Lymphocytes were gated on FCS vs SSC plot. Doublets were excluded on FSC -H vs -W plot. Dead cells were excluded as Zombie UV+. CD3 Ab: UCHT1-PE-Cy7. CD4 ab: SK3-APC-Cy7.
Data Image

Click on the image for full size data.
Publication Status

This data has not been published.
See all Reviews
ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Forgot your password? Reset Password
Request an Account