Product: Biotin anti-mouse CD1d (CD1.1, Ly-38)

Catalog No.: 123505

Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

Anti-mouse CD1d Biotin for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
Good option to stain CD1d.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse splenocytes
Brief Protocol:	Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti-CD1d Biotin (1:400). Incubate 15min at 4°C in the dark. Wash with PBS/BSA, spin down and resuspend in 50 µl staining solution containing SA-fluorescent labeled. Wash twice with PBS/BSA, resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells.
Results Summary:	CD1d is expressed by marginal zone B cells in the spleen.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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