Product: PE anti-mouse CD5

Catalog No.: 100608

J Lykken, Dr. Duke University

Clearly Marks CD5 Expression on Leukocytes (PE)
Overall:	Product Quality:	Ease of Use:
CD5-expressing splenic leukocytes were readily visualized by this antibody.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse Splenocytes
Brief Protocol:	Isolate splenocytes. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer. Stain with isotype control or mouse CD5 antibody (1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL FACS buffer containing 1.5% paraformaldehyde.
Results Summary:	CD5 expression was easily visualized on primary mouse splenocytes.
Additional Notes:

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Publication Status This data has not been published.

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