Product: APC anti-mouse CD24

Catalog No.: 101813

Javier Carrero, Assistant Professor Washington University School of Medicine

Solid CD24 Detection in Lymph Nodes
Overall:	Product Quality:	Ease of Use:
Detects both B cells and myeloid cells positive for CD24 in peripheral lymphoid organs.

Experimental Design

Application:	Flow cytometry
Cells used:	Lymph nodes
Brief Protocol:	1) Isolated cells and made single cell suspension using collagenase/DNAse I. 2) Washed cells with wash buffer (PBS, 1% bovine serum albumin). 3) Blocked Fc receptors with anti-CD16/32 (clone 2.4G2) at 500ng/mL in wash buffer at a final volume of 100 microliters per million cells. 4) Washed cells with wash buffer. 5) Resuspended cells in 100 microliters and added 0.5 microliters of antibody (1:200) per million cells. Mixed well and incubated at 4 degrees centigrade for 30 minutes. 6) Washed cells twice with wash buffer. 7) Added 2% paraformaldehyde in PBS to fix the cells. 8) Washed with wash buffer once. 9) Kept at 4 degrees centigrade until ready to analyzed (up to 24 hours). All steps done in a 96 well V-bottom plate.
Results Summary:	We detect an average of 4 logs shift in most MHC II+ cells in the lymph nodes.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

Enzymatic treatment of samples may affect your epitopes and the antibody's ability to recognize them.

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