||Measuring α-synuclein levels in conditioned medium
||Collected conditioned medium every 48 hrs (150 uL per 30,000 cells).
Diluted samples 1:3 in kit buffer.
Used white opaque plates and 100 ms integration time.
Corrected levels (pg/mL) by normalising values to the protein content in conditioned medium (i.e. values if medium at 1 ug/uL).
||Results ranged 5x10^4-5x10^7 RLU for standards and 5x10^5-2x10^7 RLU for samples, so these can be diluted even further!
||I couldn't detect α-synuclein in undiluted samples using calorimetric assays. So this works much better.
As this is a very sensitive kit, it is important to dilute samples appropriately and read with low integration times to avoid saturation of wells.