Product: PE/Cy7 anti-mouse CD45.2
Catalog No.: 109830
Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

Anti-mouse CD45.2 PE/Cy7 for Flow Cytometry
Overall: Product Quality: Ease of Use:
Good option to stain CD45.2.
Experimental Design

Application: Flow cytometry
Cells used: Mouse splenocytes
Brief Protocol: Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti-CD45.2 PE/Cy7 (1:300). Incubate 15min at 4°C in the dark. Wash twice with PBS/BSA, resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells.
Results Summary: In C57BL/6 mice lymphocytes express the congenic marker CD45.2. Upon transfer of cells bearing CD45.1 (purified from Ly5.1+/+ mice), the use of anti-CD45.2 PE/Cy7 allows to identify the recipient cells CD45.2+CD45.1-.
Additional Notes:
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