Product: PE anti-mouse IL-17A

Catalog No.: 506904

J Lykken, Dr. Duke University

Clearly Labels IL-17a+ Peritoneal Cavity Cells (PE)
Overall:	Product Quality:	Ease of Use:
Clearly labels mouse IL-17a+ peritoneal cavity leukocytes.

Experimental Design

Application:	Flow Cytometry
Cells used:	Peritoneal lavage cells
Brief Protocol:	Isolate peritoneal leukocytes and culture for 5 hours with monensin with or without added PMA and ionoymcin. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer. Permeabilize cells at room temperature for 1 hour or overnight. Wash cells in permeabilization buffer Stain with isotype control or mouse IL-17a antibody (1:100 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in permeabilization buffer. Resuspend cells in 100-250 uL PBS containing 1.5% paraformaldehyde.
Results Summary:	IL-17a+ peritoneal cells were clearly visible.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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