Product: APC anti-human CD45 |
Catalog No.: 304012 |
Khaled Waleed, Dr. University of Michigan |
Find Your Leukocytes! (APC anti-human CD45) | |||
Overall: | Product Quality: | Ease of Use: | |
Staining for a pan-leukocyte marker with confidence. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Human lung cancer single-cell homogenate |
Brief Protocol: | After obtaining a single-cell suspension, place an aliquot of cells (~300,000) into a FACS tube, add 2.5-5uL of the antibody, place on ice for 30 minutes, wash and then resuspend, ready for flow cytometric analysis. |
Results Summary: | Two clear peaks are seen: CD45+ cells are the leukocytes, CD45- events are all the non-leukocyte cells. |
Additional Notes: | Great antibody, good price, good results. |
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