Product: PE/Cyanine7 anti-human CD45 |
Catalog No.: 304015 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining PBMC with anti-human CD45 PE/Cyanine7 | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-CD45 PE/Cyanine7 works well on human PBMC. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Human PBMC |
Brief Protocol: | PBMC were isolated from blood by centrifugation over Ficoll. PBMC were washed twice in PBS and stained for 15 minutes at room temperature with the recommended dilution of anti-CD45 PE/Cyanine7 in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II. |
Results Summary: | CD45 staining was excellent. |
Additional Notes: |
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