Product: Pacific Blue™ anti-human CD3

Catalog No.: 300329

Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital

Staining PBMC with anti-human CD3 Pacific Blue™
Overall:	Product Quality:	Ease of Use:
Anti-CD3 Pacific Blue™ works well on human PBMC.

Experimental Design

Application:	Flow cytometry
Cells used:	Human PBMC
Brief Protocol:	PBMC were isolated from blood by centrifugation over Ficoll. PBMC were washed twice in PBS and stained for 15 minutes at room temperature with the recommended dilution of anti-CD3 Pacific Blue™ in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II.
Results Summary:	CD3 staining was excellent (filled histogram), as compared to unstained cells (dotted line).
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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