Product: Pacific Blue™ anti-human CD57 |
Catalog No.: 322316 |
Quirin Hammer, M Sc German Rheumatism Research Center |
Pacific Blue™ anti-human CD57 on Human NK Cells | |||
Overall: | Product Quality: | Ease of Use: | |
Bright staining of CD57 on NK cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Freshly isolated human PBMC |
Brief Protocol: | Freshly isolated human PBMC cells were washed with PBS, stained for 15 minutes at room temperature in the dark, washed two times with PBS, fixed in 2% paraformaldehyde solution for 15 minutes at room temperature in the dark, washed two times with PBS/BSA and subsequently analyzed at a BD LSRFortessa flow cytometer. Cells are gated on viable (fixable viability dye negative) CD14- CD19- CD3- CD56dim NK cells. |
Results Summary: | Clear separation of CD57+ and CD57+ NK cells. |
Additional Notes: |
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