Product: Purified anti-SNAP-25 |
Catalog No.: 836304 |
Timur Mavlyutov, Scientist UW Madison |
Very Good SNAP25 Antibody (Purified) | |||
Overall: | Product Quality: | Ease of Use: | |
Specific detection of SNAP 25 in the plasma membrane of NSC-34 cells. |
Experimental Design |
Application: | Immunocytochemistry |
Cells used: | NSC34 |
Brief Protocol: | NSC34 cells were fixed with 4% PFA for 15 min, rinsed, permeabilized with 1% Triton for 15 minutes, blocked for 1 hour in 10% normal donkey serum and stained with mouse anti-SNAP25 antibody (BioLegend #836304), diluted 1/500 for 1 hour. Secondary donkey-anti-mouse Cyanine3 conjugated antibody were applied for 1 hour. Cells were rinsed and counterstained with DAPI. Image was collected with Leica confocal microscope. |
Results Summary: | Reas signal in NSC34 cells. Thus SNAP25 is expressed in the cells. |
Additional Notes: |
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