Product: Brilliant Violet 510™ Streptavidin

Catalog No.: 405233

Julia Dickow, M.Sc. Essen University Hospital

Brilliant Violet 510™ Streptavidin
Overall:	Product Quality:	Ease of Use:
This antibody performed extremely well in staining CD8+ mouse splenocytes by flow cytometry.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse splenocytes
Brief Protocol:	Mouse splenocytes were isolated by the use of a cell strainer. Approximately 4 · 106 were used for the staining. Cells were first incubated with Biotin anti-mouse CD8 antibody for 10 minutes, were washed once and second staining with Brilliant Violet 510™ SAV was performed for 30 minutes (dilution 1:400). Both stainings were incubated in the dark and at room temperature in a total volume of 50 µl PBS, 0,1% BSA and 0,02% Natriumazid. Cells were washed once and were measured immediately.
Results Summary:	Antibody works very well for staining CD8 T cells.
Additional Notes:

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Publication Status This data has not been published.

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