Product: Purified Goat anti-mouse IgG (minimal x-reactivity)

Catalog No.: 405301

Alexander Devaux, Researcher Boston University

Highly Sensitive for ELISA (Purified Goat anti-mouse IgG (minimal x-reactivity))
Overall:	Product Quality:	Ease of Use:
This product produced signal even when diluted to 1:15000 for use as capture antibody in ELISA

Experimental Design

Application:	ELISA
Cells used:	N/A
Brief Protocol:	Diluted GAM 4053 at 1:1000, 1:5000, and 1:15000 and coated on 96 well plate with bicarbonate buffer. 75 µL used to coat wells (1 hr) Performed serial dilutions 1:5 of target epitope (mouse IgG1) starting at 2250 ng, down to 3.6 ng per well. Used cRPMI as negative control. (1 hr) Washed, then Used 2 different dilutions of detection antibody (Invitrogen cat# 62-6520), for each dilution of capture antibody. (1 hr) Washed, then added quanta blue fluorescing substrate (30 min), quenched, and read plate on fluoresence plate reader.
Results Summary:	1:1000 dilution gave best results with 1:8000 dilution of detection mab. Produced a nice log curve.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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