Product: PE anti-mouse/rat/human FOXP3

Catalog No.: 320008

jackie tung, post doc university of rochester

Antibody is Difficult to Compensate When Using 9 Colors
Overall:	Product Quality:	Ease of Use:
This antibody poorly detected Foxp3.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse T cells
Brief Protocol:	Followed BioLegend's recommended dilution. It was incubated in eBioscience permeabilization buffer. I followed the fix/perm protocol from eBioscience.
Results Summary:	For this experiment, I fixed and perm my cells. For compensation, I used eBioscience's beads. This antibody was a poor antibody to detect Foxp3.
Additional Notes:	When I was acquiring my experimental sample, most of the events was not showing within the dot plot and had to recompensate and alter the PMT voltage. A few scattered events were displaying in the Foxp3 positive gate, however I question the validity of the results.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

If you would like to troubleshoot, please feel free to contact tech@biolegend.com. In general, we would suggest using True-Nuclear Transcription Factor Buffer (424401)to detect FOXP3.

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