Product: FITC anti-human CD54 |
Catalog No.: 322720 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining PBMC with CD54 (ICAM-1) FITC | |||
Overall: | Product Quality: | Ease of Use: | |
FITC is a great choice for detecting CD54 on T cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Human PBMC |
Brief Protocol: | PBMC were isolated from blood by centrifugation over Ficoll, washed twice in PBS, and stimulated overnight with anti-CD3/CD28 beads. Cells were washed twice and stained for 20 minutes with anti-CD54 FITC (as well as CD3, CD4, CD8 antibodies). Cells were washed twice and analyzed on a BD LSR II. |
Results Summary: | CD54 staining was as expected. |
Additional Notes: |
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