At BioLegend, one way that we address reproducibility in research is through antibody validation. We guarantee antibody specificity of all of our antibody products. As knocking down or knocking out a protein is one of the most trusted ways to validate antibody specificity, we are beginning to validate many of our cell biology antibodies using knockdown or knockout models.
For this, we are testing our antibodies in Western Blot applications using in-house developed CRISPR/Cas9-mediated gene knockout/knockdown or siRNA-mediated knockdown. In addition, we are collaborating with EdiGene Inc. (a genome editing company) to provide additional CRISPR/Cas9 knockout cell lysates for testing. The data from these validation experiments will be made readily available to researchers.