If you were not able to join our activities at AAI IMMUNOLOGY2024™, we compiled our resources and presentations to make them available to you.
Scroll down to watch our workshops and download PDFs of in-booth talks and poster abstracts presented at the show. Also, view pictures of sponsored events and learn more about our expanded capabilities now powered by Revvity’s portfolio.
Zayda Piedra, PhD
Scientist II, Scientific Applications Group, BioLegend
To fully characterize cell function and ultimately understand pathological conditions, technologies evolve to provide more efficient ways to interrogate biological systems. One such tool that has emerged is spectral flow cytometry. With capacity to multiplex >40 parameters, the availability of so many reagents comes with challenges, making it difficult to find the best conjugates to use and increasing the complexity of the optimization process. Here we discuss BioLegend’s strategies to build complex panels, including backbone combinations that leave open channels for easy expansion. Thus, with our extensive portfolio and experience, BioLegend can help your research with either pre-optimized panels or a customized design.
Josh Koenig, PhD
Assistant Professor, McMaster University, Immunology Research Centre
The pathways responsible for the emergence of IgE antibodies that drive food allergy remains poorly understood. Using a model of the early stages of food allergy, Koenig et al. found that a single gavage of allergen with cholera toxin adjuvant failed to sensitize mice, but re-exposure to the allergen alone even 1 year later caused sensitization and anaphylaxis upon allergen challenge. The first intragastric exposure induced CD44hi lineage-ambiguous CD4+ T cells but failed to activate B cells. Allergen re-exposure drove Tfh differentiation and IgE production de novo through naïve B cells dependent on IL-4 and CD40/CD40L ligation. Koenig's data implicated memory CD4+ T cells in initiating IgE production and holding IgE memory.
Frank Harrison
Portfolio Manager, Proteogenomics, BioLegend
Multiomic single-cell analysis is a powerful tool to analyze and understand cell function. In this talk, we will discuss the most recent additions to our TotalSeq™ antibody portfolio, as well as the newest applications in multiomic single-cell analysis powered by our oligo-antibody conjugates. From detection of intracellular molecules to new, focused, lyophilized panels and more, we will discuss an update of the latest technology from our labs and most recent products available to support your research.
Rodney Nash, PhD
Scientific Officer, Revvity
Flow cytometry, NGS, and single-cell analysis have undoubtedly advanced the field of immunology. Protocol optimization enables data reproducibility and assay consistency, critical for delicate immune cells of limiting quantities. We present method comparison work using a bead mill for sample disaggregation into single-cell suspension versus a manual, cell-strainer method in this proof-of-concept study with mouse spleen. Data is shown for cell count, cell viability, and immune cell marker populations via flow cytometry.
We congratulate the two winners of the $200 Amazon.com Gift Card: Indira Neeli from the University of Tennessee Health Science Center and Carlos Andres Oroya Lazo from the Hospital Maisonneuve-Rosemont. Thanks for visiting our booth and learning more about our expanded solutions!
You can view and download them by clicking their images
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Title
Study of human KIR family molecules with novel monoclonal antibodies
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Title
Developing a multiplex assay to monitor apoptosis and necrosis using the Cellaca™ PLX Image Cytometer
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Cellaca® PLX
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Title
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies
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Cellaca® PLX
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Title
Alternative cell counting and viability detection using NexGreen/PI fluorescent stain on multiple low-and high-throughput image cytometry platforms
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Cellometer® Auto2000
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Cellometer® Spectrum
Cellaca® MX
Cellaca® PLX
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Title
Characterizing the health of transduced CAR-T cells using high-throughput image cytometry
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Cellaca® PLX
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Title
Development of a T cell activation panel using image cytometry in rheumatoid arthritis patient samples
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Cellaca® PLX
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The following are a few highlights from AAI IMMUNOLOGY2024™, held in Chicago, Illinois, from May 3-7.
For their contributions to the immunology field, BioLegend selected 3 winners for the AAI IMMUNOLOGY2024™ Travel Award. We also proudly sponsored the AAI-BioLegend Herzenberg Award and the Lefrancois-BioLegend Memorial Award. This year, these awards were granted to John C. Cambier, PhD, Professor, from the University of Colorado, School of Medicine, and Vini John, PhD, Postdoctoral Research Scholar, from the Washington University, School of Medicine, respectively. Congratulations to all the winners!
John C. Cambier, PhD
Professor, University of Colorado, School of Medicine
Vini John, PhD
Postdoctoral Research Scholar, Washington University, School of Medicine
Our current round of travel awards has ended, but check back frequently to see the new funding opportunities we are providing to alleviate travel costs.
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Solutions |
Cellular analysis |
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Flow cytometry products |
We provide thousands of antibody and fluorophore combinations to build the ideal panel to phenotype and understand your cells. |
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TotalSeq™ oligo- conjugated antibodies for multiomics analysis |
For single-cell and bulk multiomics, we provide TotalSeq antibodies conjugated to oligonucleotide barcodes to add a new layer of protein detection to assays like scRNA-seq. |
TotalSeq™ oligo-conjugated antibodies for multiomics l BioLegend |
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Solutions |
Sample preparation |
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Omni Bead Ruptor Elite™ bead mill homogenizer |
The Omni Bead Ruptor Elite is one of today’s most advanced and powerful bead mill homogenizers. Uniquely designed to dissociate, grind, lyse, and homogenize the most challenging samples, it streamlines protocols to save you time and produce repeatable results. |
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MojoSort™ |
Our MojoSort cell separation system of magnetic bead-conjugated antibodies, buffers, and magnets provides a simple method for the isolation and purification of cells in heterogeneous populations. |
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Solutions |
Cell counting and imaging cytometry |
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Cellometer™ and cell counting reagents |
Cellometer image cytometer utilizes dual-fluorescent and brightfield imaging to quickly identify and count individual cells. Cellometer reagents are optimized to perform fluorescence-based cell counting and viability assays used to measure percent viability and enumerate live/dead cells. |
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Cellaca™ PLX and reagents |
The Cellaca PLX system with Matrix analysis software and dedicated reagents and consumables provides a benchtop solution for accurate measurements of small sample volumes to easily perform rapid subpopulation analysis for downstream processing. |
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Solutions |
Cell culture and bioprocessing |
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GMP and Cell-Vive™ solutions |
To ensure quality at every step of the bioprocessing workflow, we offer recombinant proteins, functional antibodies, and cell culture reagents produced under Good Manufacturing Practices (GMP) for ex vivo cell processing and downstream manufacturing. |
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Recombinant proteins |
We drive cell fates by providing stable, bioactive human, mouse, and rat recombinant proteins including cytokines, growth factors, and chemokines. |
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Solutions |
Immunoassays |
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LEGENDplex™ |
Use a flow cytometer to measure multiple soluble cytokines, chemokines, and proteins simultaneously with our pre-defined or customized LEGENDplex multiplex panels. |
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ELISA kits and sets |
For researchers looking to quantify individual protein targets, we provide ELISAs in multiple formats that are compatible with biological samples like cell culture supernatant, serum, and plasma. |
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HTRF™ assay kits |
In recent years, steady improvements have made HTRF the gold standard for time-resolved FRET. It has become rapid, homogeneous, easy to use and automate, allowing miniaturization and assay development. |
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AlphaLISA™ assay kits |
Fast results, high sensitivity, large dynamic range, and a compatibility with many sample types make Alpha technology an optimal choice for moving your research forward. |
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VICTOR® Nivo™ |
The VICTOR Nivo system packs all popular detection modes into the industry's smallest multimode reader footprint; the perfect microplate reader for everyday biochemical and cell-based assays. |
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Solutions |
RNAi, CRISPR, and base editing technology |
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Dharmarcon™ CRISPR reagents |
With our Revvity Dharmacon™ Edit-R™ CRISPR-Cas9 reagents, researchers can make targeted, specific edits to any gene in practically any cell type. |
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Dharmacon™ RNAi reagents |
Revvity’s Dharmacon reagents have pioneered the development of novel siRNA, shRNA, and microRNA technology for nearly 30 years, offering a broad portfolio of trusted synthetic and expressed reagents for highly specific and efficient gene silencing. |
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Pin-point™ base editing platform |
The Pin-point base editing system offers the specificity of base editing combined with the potential for multiplex gene editing capabilities, making it an attractive technology for both cell and gene therapies. |
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Solutions |
Microscopy and spatial biology |
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Spatial biology imaging reagents |
Enter a new era of spatial biology with BioLegend’s reagents for traditional imaging applications, such as IHC/ICC, or advanced techniques involving multiplexed imaging of dozens of targets. |
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In vivo imaging systems and reagents |
Our in vivo imaging solutions include the leading IVIS™ optical platform, Quantum™ GX3 microCT system, and Vega™ ultrasound scanner, as well as a comprehensive portfolio of IVISbrite™ bioluminescent and IVISense™ fluorescent reagents optimized for your research applications. |
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Cellular imaging platforms |
Create deep phenotypic insights from your most demanding assays with our leading Opera Phenix™ Plus and Operetta™ CLS high content imaging platforms or rapidly assess cell viability with our Celigo™ image cytometer. |
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PhenoPlate™ microplates |
PhenoPlate microplates have been engineered to deliver superior images and the highest quality data for high-content applications, complementing our proven high content screening (HCS) instruments, reagents, and image analysis software. |
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PhenoVue™ cellular imaging reagents |
Our suite of PhenoVue cellular imaging reagents—including cell painting kits, organelle and cell compartment stains, fluorescent labeled secondary antibodies, and cell function reagents— are optimized for HCS to streamline your workflow. |
Research use only. Not for use in diagnostic procedures.
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