GoInVivo™ features

  • Flow cytometry quality testing
  • Super low endotoxin, ≤ 1.0 EU/mg
  • Low aggregation, < 3%
  • Pathogen-free
  • Bulk sizing (5 mg, 25 mg, 50 mg, 100 mg, 500 mg, 1 g sizes)
  • Concentration between 1 mg/ml and 3 mg/ml
  • Custom sizes available
  • Sodium azide and preservative-free
In addition, some of our antibodies are validated in functional assays, including anti-human PD-1 (clone EH12.2H7). All GoInVivo™ reagents will be validated by functional assays.
Anti-human PD-1 inhibits the binding of PD-L1. The graphs show two sets of data. First, recombinant human PD-L1-Fc binds to immobilized human PD-1-Fc that has been coated overnight on a 96-well plate (Cyan lines). PD-1-Fc was coated at 1 µg/mL, and increasing concentrations of PD-L1-Fc (cyan diamonds) or paired PBS wells (cyan circles) were incubated overnight. The bound recombinant PD-L1-Fc was detected using biotin anti-human PD-L1 and Avidin HRP, followed by TMB. After verifying that PD-L1-Fc binds to immobilized PD-1-Fc, a second experiment was performed in which the coated PD-1-Fc was pre-incubated with increasing concentrations of anti-human PD-1 (clone EH12.2H7, purple squares) or isotype control (clone MOPC-21, purple circles), followed by incubation with a fixed concentration of PD-L1 (1 µg/mL). Clone EH12.2H7 inhibits PD-1/PD-L1 interaction in a dose dependent manner.