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KIRAVIA Multimers

Introducing the KIRAVIA Dyes™, a brand new fluorescent chemistry to advance applications in multicolor flow cytometry and beyond. This class of dyes employs a unique organic backbone that separates fluorophores to minimize quenching effects, thus allowing optimal and higher fluorophore to protein (F:P) ratios, surpassing what is possible with direct conjugations of single fluorophore like FITC.

KIRAVIA Blue 520™

Brightness: 4
Ex Laser: 488 nm
Ex Max: 488 nm
Em Max: 520 nm
Filter Used: 530/30
Molecular Weight: 8 kDa
Recommended Application(s):
Flow Cytometry

Fluorophore at a Glance:

  • An upgrade over FITC in terms of brightness.
  • A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™.
  • Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression.
 

View all KIRAVIA Blue 520™ Products.
 

Learn more about KIRAVIA Dyes™.

Equivalent fluorophores comparison

Amazing Resolution

Anti-human CD4 (clone SK3), conjugated to FITC (red), Alexa Fluor® 488 (blue), BD Horizon™ Brilliant Blue 515 (green), or KIRAVIA Blue 520™ (purple) was used to stain human lysed whole blood.

(Left) Anti-human CD3 (clone UCHT1) BV421™ and anti-human T-bet (clone 4B10) KIRAVIA Blue 520™ staining on human PBMCs. (Right) Titration curve with FITC or KIRAVIA Blue 520™ conjugated to anti-T-bet antibody.
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