Video Guide: Flow Cytometry

Thank you for taking an interest in our videos. You can read through the descriptions for our videos below or find additional videos in our Video Library.

• Isolating PBMCs with Lymphopure™: Watch to learn how you can reliably isolate mononuclear cells from whole blood in just 5 easy steps using Lymphopure™ density gradient medium.
   
• Surface and Intracellular Cytokine Staining for Flow Cytometry: This video provides a list of the reagents required and the steps needed to perform flow cytometry staining, covering fixation, permeabilization, and both surface and intracellular staining with antibodies.
   
• True-Nuclear™ Buffer Set Webinar: In this mini-webinar, scientist David Soper, discusses the advantages of BioLegend’s True-Nuclear™ Transcription Factor Buffer Set over the FOXP3 Buffer Set.
   
• Intracellular Phospho Staining for Flow Cytometry: Protein phosphorylation is a critical step in a wide variety of signaling pathways. Watch this video to learn how to use True-Phos™ Perm Buffer to detect these phosphorylated targets by flow cytometry.