MojoSort™ Human CD8 Nanobeads

Pricing & Availability
Other Names
Human CD8 positive selection kit, CD8 positive, human CD8 depletion kit, CD8 depletion
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Human_CD8_Nanobeads_1_010218
PBMCs was prepared to isolate or deplete CD8+ cells using the MojoSort™ Human CD8 Nanobeads. Cells were stained with anti-human CD8 (clone RPA-T8) PE. Dead cells were excluded by 7-AAD.
  • Human_CD8_Nanobeads_1_010218
    PBMCs was prepared to isolate or deplete CD8+ cells using the MojoSort™ Human CD8 Nanobeads. Cells were stained with anti-human CD8 (clone RPA-T8) PE. Dead cells were excluded by 7-AAD.
  • Human_CD8_Nanobeads_2_010218
Cat # Size Price Quantity Avail. Save
480107 10 tests $150.00
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480108 100 tests $450.00
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Description

Human CD8+ cells are either selected or depleted by incubating the sample with the directly conjugated CD8 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD8+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480107:

  • 1 vial containing 100 µl of Human CD8 Nanobeads

For Cat# 480108:

  • 1 vial containing 1 ml of Human CD8 Nanobeads

Product Details

Reactivity
Human
Formulation
Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Antibody-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of Human CD8 Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of human CD8+ cells from peripheral blood mononuclear cells (PBMCs).

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

To find out the dilution to use this product in separation columns please download the CoA.

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 01/05/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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