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- Regulatory Status
- RUO
- Other Names
- Human Bone Formation Panel (4-plex)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Sensitivity is defined as the mean minimum detectable concentration (MDC) established by applying a 5-PL curve-fitting algorithm to assay curves run in both serum/plasma matrix (n = 13) or cell culture media (n = 11) in the LEGENDplex™ Data Analysis Software. -
Representative LEGENDplex™ Human Bone Metabolism Panel 1 standard curves. Data were acquired using a dual laser BD FACSCalibur™ flow cytometer. -
Synovial fluid samples from patients with psoriatic arthritis (PsA, n = 24) or rheumatoid arthritis (RA, n = 22) were quantified using the LEGENDplex™ Human Bone Metabolism Panel 1. Data are presented as Mean + SD. Data were provided courtesy of Dr. Erik Lubberts, Erasmus University Medical Center, Rotterdam, Netherlands.
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Bone metabolism is a complex equilibrium between bone formation and resorption regulated through numerous hormones, steroids, growth factors, and cytokines. Bone formation is mediated through specialized cells called osteoblasts, while resorption is carried out via osteoclasts. Bone metabolism is a constant, life-long process that exists in balance. Disruptions to the homeostasis between formation and resorption results in diseases such as: osteoporosis, osteopetrosis, Paget’s disease, rickets, and renal osteodystrophy.
The LEGENDplex™ Human Bone Formation Panel (4-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 4 key targets involved in bone formation including OPG, ALPL, Leptin, and BMP-2. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
Kit Contents
- Kit Contents
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- Setup Beads 1: FITC Beads (1 ml)
- Setup Beads 2: PE Beads (1 ml)
- Setup Beads 3: Raw Beads (1 ml)
- Capture Beads (varies)
- Detection Antibodies (3.5 ml)
- Standard Cocktail, Lyophilized (1 vial)
- SA-PE (3.5 ml)
- Matrix C, Lyophilized (1 vial)
- Reconstitution Buffer (1 ml)
- Assay Buffer (25 ml)
- Wash Buffer (25 ml)
- Plate Sealers (4 sheets)
- Data Analysis Software Dongle
- Filter Plate
- Manual
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
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- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Aluminum foil
- Absorbent pads or paper towels
- Sonicator
- Plate Shaker
- Tabletop centrifuge
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
Antigen Details
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines, Enzymes and Regulators, Growth Factors
- Gene ID
- 4982 View all products for this Gene ID 249 View all products for this Gene ID 3952 View all products for this Gene ID 650 View all products for this Gene ID
- App Abbreviation (DOES NOT SHOW ON TDS):
- Multiplex - Panel
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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