LEGEND MAX™ Human PlGF ELISA Kit with Pre-coated Plates

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Other Names
PlGF, PLGF, Placental Growth Factor
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Human_PlGF_ELISA_BA_112017
  • Human_PlGF_ELISA_BA_112017
Cat # Size Price Quantity Save
446007 1 Pre-coated Plate $425.00
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446008 5 Pre-coated Plates $1,600.00
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Description

Placental growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors. Four alternatively-spliced isoforms have been identified: PlGF-1, PlGF-2, PlGF-3, and PlGF-4. All isoforms are expressed in placental tissues, with PlGF-2 being expressed specifically during early placental development. PlGF-1 and PlGF-3 are considered diffusible isoforms, while PlGF-2 and PlGF-4 each contain a unique heparin binding domain that may increase associations with cell membranes. In addition to placental tissues, PlGF is expressed in the microvasculature, bone marrow, umbilical vein endothelia, uterine keratinocytes, and NK cells. PlGF binds to both VEGF-R1 and neuropilin, but not VEGF-R2 and induces monocyte activation, migration, inflammatory cytokine production. PlGF plays a key role in maternal vascular function during pregnancy, with circulating levels reaching a peak mid-gestation. It can be used as a marker for pregnancy-associated hypertensive disorders such as pre-eclampsia. High levels of PlGF expression have also been linked to tumorigenesis; specifically PlGF-1 has been identified in both colon and mammary carcinomas. 

The BioLegend LEGEND MAX™ Human PlGF ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human PlGF capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human PlGF antibody. This kit is specifically designed for the accurate quantitation of human PlGF from serum, urine, cell culture supernatant and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human PlGF Pre-coated 96-well Strip Microplate
  • Human PlGF Dectection Antibody
  • Human PlGF Standard
  • Matrix A (for serum samples only)
  • Avidin-HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Sensitivity
0.39 pg/mL
Standard Range
3.1 - 200 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

If the kit says it is in 5/10/15 plate format, what does it mean?

It means that kit has enough reagents for 5, 10 or 15 plates depending on which catalog number you have ordered.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 0    Revision Date: 12/13/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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