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ELISA MAX™ Deluxe Set Mouse IL-25 (IL-17E)

Pricing & Availability
Regulatory Status
RUO
Other Names
IL25, IL-17E, IL17E, Interleukin 25, Interleukin 17E
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  • ELISA-MAX_Mouse_IL-25_Antibody_011520.PNG
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447104 5 plates $370
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Description

IL-25, also known as IL-17E, is a pro-inflammatory cytokine encoded by the IL25 gene located on chromosome 14. The IL-17 family consists of IL-17A, IL-17B, IL-17C, IL-17F, IL-17A/F, and IL-17E. The IL-17 family has a similar protein structure with four conserved cysteine residues and two disulfide bonds. IL-25 expression is widely distributed and can be produced by a variety of cells and tissues. IL-25 induces T helper 2 (Th2) expansion and production of cytokines IL-4, IL-5, and IL-13. This is important in eosinophil expansion, regulating gut immunity, and in the response to helminth parasites.

 

IL-25 has been demonstrated to play a role in allergy and asthma. IL-25 levels are positively correlated with the severity of allergenic rhinitis in patients. Blocking IL-25 has been proposed as an allergy treatment in combination with other therapies. IL-25 has also been associated with the pathogenesis of rheumatoid arthritis and is frequently upregulated in the serum and synovial fluid of patients and in mouse models of rheumatoid arthritis.

 

BioLegend's ELISA MAX™ Mouse IL-25 (IL-17E) ELISA MAX™ Deluxe Set is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate. The plate is pre-coated with a monoclonal mouse capture antibody. The detection antibody is a biotinylated mouse monoclonal antibody. This kit is specifically designed for the accurate quantitation of mouse IL-25 from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  1. Mouse IL-25 (IL-17E) ELISA MAX™ Capture Antibody (200X)
  2. Mouse IL-25 (IL-17E) ELISA MAX™ Detection Antibody (200X)
  3. Mouse IL-25 (IL-17E) Standard
  4. Avidin-HRP (1000X)
  5. Substrate Solution D
  6. Coating Buffer B (5X)
  7. Assay Diluent D (1X)
  8. Assay Diluent A (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
1.31 ± 0.43 pg/mL
Standard Range
12.5 - 800 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Type
Basophils, Dendritic cells, Eosinophils, Epithelial cells, Macrophages, Mast cells, T cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
IL-25
Specificity Alt (DOES NOT SHOW ON TDS):
IL-25
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA
UniProt
View information about IL-25 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 01/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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